See Table Table22 for details.Table 2Characteristics of control groupDatasetsData were obtained from standardized super-syringe maneuvers [32] (Figure (Figure1).1). Briefly, during the automatically Ganetespib operated maneuvers, the ventilator repetitively applied volume steps of 100 mL, with an inspiratory airflow rate of 558 �� 93 mL/sec for the ARDS group and 470 �� 95 mL/sec for the control group up to a maximum plateau pressure of 45 cmH2O. At the end of each volume application, airflow was interrupted for three seconds.Figure 1Super-syringe maneuver. Representative time-series for standardized super-syringe maneuvers obtained from one acute respiratory distress syndrome (ARDS) and one patient with healthy lungs (control). Volume steps of 100 mL were repetitively applied up …

Data analysisAll analyses and model simulations were carried out using the Matlab? software package Version R2006b (The MathWorks?, Natick, MA, USA).Model representationWe used an electrical analog of a spring-and-dashpot model [19,21] (Figure (Figure2)2) consisting of two components: (1) A Newtonian airway resistance (R) and a static compliance of the respiratory system (Cst) and (2) the electrical analog of a resistive dashpot (Rve) and an elastic spring (Cve) as resistance and compliance of the component which is modeling viscoelastic behavior. The time constant of the viscoelastic component (��ve) quantifies the stress relaxation dynamics of the system and is determined by the product of Rve and Cve [see Additional file 1].Figure 2Lumped parameter model. Electrical circuit analog to the spring-and-dashpot model.

R denotes the Newtonian airway resistance and Cst the static compliance. Rve and Cve are the resistance and the compliance of the viscoelastic component, respectively. …Parameter estimationFor each volume step i within each super-syringe maneuver, the parameters Ri, Cist, Rive and Cive, were estimated by fitting the model via a multiple regression analysis to the time-series data (Figure (Figure3)3) [see Additional file 1].Figure 3Flow interruption technique. (a) Respiratory flow and (b) pressure Prs time-series of one 100 mL volume step including the phases of volume loading ( >0 mL/sec) and stress relaxation ( = 0 mL/sec during occlusion interval). (a) Labeled points …Impedance analysisImpedance analysis was performed with respect to dependence on respiratory frequency for four categories: ARDS group at low (7.

5 cmH2O) and high (42.5 cmH2O) plateau pressure, and control group at the same low and high plateau pressure. For each category, the parameters R, Cst, Rve and Cve were determined and inserted into the model. For each parameterized model, a Anacetrapib Bode magnitude plot was drawn.Data presentation and statistical evaluationFor data presentation, the estimated values of the model parameters were linearly interpolated in steps of 2.